Molecular Characterisation of Cryptosporidium parvum among Cattle and Cattle Handlers from Tripura (India) and the Associated Risk Factors

Background: Cryptosporidium parvum is major protozoan parasite of both animals and humans with zoonotic significance. There a paucity information on its occurrence associated risk factors in North Eastern states, India. The present study aimed at the molecular characterization from cattle handlers West district Tripura associates analysis. Methods: Faecal samples were randomly collected (100 each), district, Tripura. subjected to sheather’s sucrose flotation, modified Zeihl Neelsen (mZN) staining PCR assay targeting 18S SSUr-RNA gene for detection Cryptosporidium. polemerised chain reaction- Restriction length polymorphism (PCR-RFLP) was done detect spp. C. genotypes. Association epidemiological variables relation host environment studied comparative Result: incidence 16 per cent 7 handlers, respectively. characterisation based PCR-RFLP analysis revealed 14 two andersoni three handlers. All one belonged bovine genotype (genotype II) other human I). Cryptosporidiosis strongly younger age group diarrhoeic faecal consistency significantly (p less than 0.01) higher occurrence. However, 0.05) predominance monsoon season disease observed sex not